www.biorxiv.org


A team at Shift Bioscience employs a novel single-cell transcriptomic clock (AC3) to screen 1,500 genes, discovering SB000 as a single-factor intervention that reverses transcriptomic and epigenetic aging in fibroblasts and keratinocytes without activating pluripotency, paving the way for safe rejuvenation therapies.

Key points

  • AC3 single-cell transcriptomic clock screens 1,500 ORFs to identify rejuvenation factors.
  • SB000 expression reduces transcriptomic age by ~4.5 years in fibroblasts and keratinocytes without pluripotency.
  • SB000 reverses multiple epigenetic clocks and increases global CpG methylation by ~3%, preserving cell identity.

Why it matters: SB000 decouples cell rejuvenation from pluripotency, offering a safer, single-gene route to reverse aging across diverse tissues.

Q&A

  • What is SB000?
  • How does the AC3 transcriptomic clock work?
  • Why avoid pluripotency for rejuvenation?
  • What evidence shows SB000 reverses epigenetic aging?
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A single factor for safer cellular rejuvenation